A comprehensive surgical protocol for ventral midline hysterectomy, ovariectomy, and ovariohysterectomy procedures specific to the mouse model is presented, emphasizing its efficacy and low complication rates.
The article outlines surgical techniques for ventral midline hysterectomy and ovariectomy procedures on mice, detailing the methodology and results of 255 surgeries with low post-operative complications.
Conducted by researchers affiliated with St. Joseph’s Hospital and Medical Center, with support from various foundations and institutions.
The study provides details on surgeries performed and data collected within the time frame stated, particularly involving mice aged 19-21 weeks.
The research was conducted at St. Joseph’s Hospital and Medical Center.
These procedures are necessary to explore the physiological impacts of ovariectomy and hysterectomy to inform studies on conditions akin to human menopause and other disorders.
Detailed methods include positioning the mice, making incisions, and conducting the surgeries using stereomicroscopic techniques.
This study shows the methodology is easily reproducible with minimal complications, showcasing innovative surgical techniques for small animal models.
“Despite their small size and the manipulation of several ... this procedure is easily reproducible and well-tolerated in mice.”
“We found ... the procedure is easily reproducible and well-tolerated ... with a relatively low attrition rate of 1.18%.”
“A comprehensive description of three surgical procedures ... using a midline partial laparotomy approach.”
1. Introduction: Introduce the significance of the study, emphasizing the importance of developing effective surgical techniques for small animal models; highlight the major findings and low complication rates.
2. Background: Background on the use of the murine model for translational research and the relevance of ovariectomy and hysterectomy to reproductive health studies.
3. Methodology and Discovery: Describe the detailed surgical protocol, emphasizing the positioning of the mice and the stereomicroscopic techniques employed for precision.
4. Findings and Implications: Present the results of the surgeries, discussing the success rate, post-operative care, and the discovered attrition rate; highlight the broader relevance of these findings in medical research.
5. Conclusion: Summarize the benefits of the newly established surgical protocols and potential future applications for research involving murine models.